The oxidase test seems like a good option to our project. However, there are concerns about the toxicity of the main reagent and its shelf life (16 weeks).
Moreover, bacteria from the staphlococcus genus are negative for the oxidase test and cannot therefore be identified this way.
Perhaps we should consider a set of three tests which could placed in strategic places in the catheter. Some ideas are:
*Indole test: performed on
bacterial species to determine the
ability of the organism to split indole from the amino acid tryptophan.
ability of the organism to split indole from the amino acid tryptophan.
Key reagent: p-dimethylaminobenzaldehyde
indole (+) bacteria: Aeromonas hydrophilia, Aeromonas punctata, Bacillus alvei, most Citrobacter sp., Edwardsiella sp., Escherichia coli, Flavobacterium sp., Haemophilus influenzae, Klebsiella oxytoca, Proteus sp. (not P. mirabilis), Plesiomonas shigelloides, Pasturella multocida, Pasturella pneumotropica, Streptococcus faecalis, and Vibrio sp.
*Citrate test: detects the ability of an organism to use citrate as the sole source of carbon and energy. Bacteria are inoculated on a medium containing sodium citrate and a pH indicator such as bromothymol blue.
citrate (+) bacteria: Citobacter, Enterobacter, Klebiella
-Thais
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